3rd CryoEM Symposium

3rd Annual Southern California Cryo-Electron Microscopy Symposium

The 3rd Annual Southern California Cryo-Electron Microscopy Symposium will be held on Friday, October 26th, 2018 at The Auditorium at The Scripps Research Institute in La Jolla, bringing together electron microscopists in Southern California focused on studying the fundamental mechanisms of biological systems.

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Applications for talks are no longer being accepted.
Those wishing to present a poster MUST register by October 17th.
Registration will close October 19th.
Program
8:00 AM - 9:00 AM
Breakfast, Registration, and Poster Setup
9:00 AM - 10:20AM
Talks (15 + 5 min)
Tomography and Cellular Studies
9:00 - 9:20 Stephen Carter – CalTech – Correlated cryogenic fluorescence microscopy and electron cryotomography shows that TRIM5α forms hexagonal nets near autophagic organelles
9:20 - 9:40Jiayan Zhang – UCLA – In situ structures of HCMV gB captured at different states by cryoET
9:40 - 10:00Mohammed Kaplan – CalTech – The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type
10:00 - 10:20Kanika Khanna – UCSD – Visualizing assembly of nucleus-like structure in bacteria by in situ cryo-electron tomography
10:20 AM - 10:50 AM
Coffee Break, Vendor Booths, and Poster Setup
10:50 AM - 12:30 PM
Talks (15 + 5 or 10 + 2 min)
Technological Advancements
10:50 - 11:10Mengyu Wu – TSRI – Pushing single-particle cryo-EM to the theoretical size and resolution limits at 200 keV
11:10 - 11:30Johan Hattne – UCLA – Analysis of global and site-specific radiation damage in cryo-EM
11:30 - 11:42Sriram Aiyer – Salk – Implications for high-resolution cryo-EM structure determination using an AAV2 capsid variant
11:42 - 11:54Indrajit Lahiri – UCSD – Using streptavidin affinity grids to overcome preferred orientation
11:54 - 12:06Charles Bowman – TSRI – Leveraging computational infrastructure for rapid and efficient high-resolution single-particle cryo-EM
12:06 - 12:18Calina Glynn – CalTech – Preferred structural polymorphisms in the β2α2 loop of mammalian prion proteins revealed by MicroED
12:18 - 12:30Jessica Rabuck-Gibbons – Salk/TSRI – Refining 3D classification parameters for compositional heterogeneity
12:30 PM - 2:00 PM
Lunch, Posters, and Vendor Booths/Talks
1:00 PM - 2:00 PM
Vendor Talks - Dell/Nvidia, Direct Electron, Gatan, Jeol, ThermoFisher Scientific
2:00 PM - 3:40 PM
Talks (15 + 5 or 10 + 2 min)
Single-Particle Applications
2:00 - 2:20 Jennifer Kefauver – TSRI – Structure of the human volume regulated anion channel
2:20 - 2:40 Andres Hernandez – TSRI – Structures of the substrate-engaged 26S proteasome reveal the mechanisms for ATP hydrolysis-driven translocation
2:40 - 2:52 Lukas Susac – UCLA – Structure of telomerase with telomeric DNA
2:52 - 3:04 Gabriel Ozorowski – TSRI – CryoEM structure-guided design of a new class of small molecule fusion inhibitors that target a pocket near the fusion peptide of HIV-1 Env
3:04 - 3:16 Rick Baker – UCSD – NECAPs are conformational sensors of the AP2 clathrin adaptor complex
3:16 - 3:28 Colby Sandate – TSRI – The pore loops of the microtubule severing enzyme spastin form an ordered network upon substrate engagement
3:28 - 3:40 Chi-Min (Mimi) Ho – UCLA – Malaria parasite translocon structure and mechanism of effector export
3:40 AM - 4:10 PM
Coffee Break, Vendor Booths, and Posters
4:10 PM - 5:00 PM
Keynote talk (40 + 10)
Keynote
Tamir Gonen – UCLA/HHMI –MicroED: conception, practice and future opportunities
5:00 PM - 5:10 PM
Closing Remarks
5:10 PM - 7:00 PM
Reception, Posters, and Vendor Booths
Venue

Built in 1995, the three-building complex including The Auditorium at The Scripps Research Institute (TSRI) was lauded by New York Times architecture critic Herbert Muschamp as a “magnificent piece of work” and cited by Time magazine as one of the best designs of 1996. The complex also received the American Institute of Architects’ National Honor Award and an SDG&E’s Savings by Design Energy Efficiency Integration Award.

Designed by the architectural studio Tod Williams Billie Tsien & Associates of New York City, working with local architect Joseph Wong Design Associates of San Diego, every element of the complex’s 56,000 square-foot-area—materials, buildings and landscape—shapes a coherent and serene environment in which world-class scientists pursue the biomedical breakthroughs that lay the foundation for improving human health.

Directions
  • From Interstate 5, exit at Genesee Avenue and go west for about 0.8 miles to the second stoplight
  • Turn right (north) onto John Jay Hopkins Drive
  • Turn left at the second opportunity (about 0.1 miles) into the institute's driveway; the gates may be raised and you may park in that lot for an auditorium event

Organizers

  • David DeRosier
  • Professor of Biology, Emeritus
  • Brandeis University
  • Mark Herzik
  • Postdoctral Fellow (Lander Lab)
  • The Scripps Research Institute (current)
  • Assistant Professor of Chemistry and Biochemistry
  • University of California, San Diego
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